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Teenage Mutant Ninja Turtles: Mutant Mayhem is a 2023 American animated superhero film based on the Teenage Mutant Ninja Turtles characters created by Peter Laird and Kevin Eastman. A reboot of the Turtles film series, it was directed by Jeff Rowe and co-directed by Kyler Spears, from a screenplay and story written by Seth Rogen, Evan Goldberg, and Rowe, with Dan Hernandez and Benji Samit also credited to the former and Brendan O'Brien to the latter. The film stars an ensemble voice cast with Micah Abbey, Shamon Brown Jr., Nicolas Cantu, and Brady Noon as the titular team, alongside Ayo Edebiri, Maya Rudolph, John Cena, Rogen, Rose Byrne, Natasia Demetriou, Giancarlo Esposito, Jackie Chan, Ice Cube, and Paul Rudd. Mutant Mayhem follows the Turtles, who, with the help of their new ally April O'Neil, go on a hunt for and face off against a mysterious crime boss, Superfly, and his gang of mutants.

Plans for an animated Turtles film with involvement by Rogen began in 2018. In June 2020, the project was officially announced, with Rogen, Goldberg, and James Weaver producing through their company, Point Grey Pictures, and Rowe as director. Seeking to explore the teenage aspect of the Turtles, the filmmakers drew inspiration from teenage coming-of-age films. The animation was provided by Mikros Animation and Cinesite, and was primarily influenced by school notebook sketches. In addition to a score composed by Trent Reznor and Atticus Ross, the film features various classic East Coast hip-hop songs.

Mutant Mayhem was screened as a work-in-progress at the Annecy International Animation Film Festival on June 12, 2023, and was released by Paramount Pictures in the United States on August 2, 2023. It grossed $180.5 million worldwide and received positive reviews for its performances, screenplay, and stylized animation; several critics considered it the best Turtles film. The film was nominated for Best Animated Feature at the 29th Critics' Choice Awards, 51st Annie Awards (in addition to five other nominations), and 35th Producers Guild of America Awards among numerous other accolades. A sequel is set to release in theaters on September 17, 2027, while a follow-up television series, Tales of the Teenage Mutant Ninja Turtles, debuted on the streaming service Paramount+ in August 2024.